Cassette Ovulation Test Instructions
WHEN TO BEGIN TESTING
First, you must determine the length of your menstrual cycle. This is the number of days from the first day of your menstrual period to the day before your next period begins. Please refer to the chart to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day over a 5 day period, or until the LH surge has been detected.
| Your Cycle Length | Start To Test On |
| 21 days | Day 6 |
| 22 days | Day 6 |
| 23 days | Day 7 |
| 24 days | Day7 |
| 25 days | Day 8 |
| 26 days | Day 9 |
| 27 days | Day 10 |
| 28 days | Day 11 |
| 29 days | Day 12 |
| 30 days | Day 13 |
| 31 days | Day 14 |
| 32 days | Day 15 |
| 33 days | Day 16 |
| 34 days | Day 17 |
| 35 days | Day 18 |
| 36 days | Day 19 |
| 37 days | Day 20 |
| 38 days | Day 21 |
URINE COLLECTION
Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day. The best time to collect your urine is between 10am and 8pm. Collect urine at about the same time each day. Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting the LH surge.
TEST PROCEDURE
1. Allow the sealed LH Midstream test pouch to reach room temperature (18-30°C). Remove the LH Cassette device from the sealed pouch.
2. Draw the urine sample into the urine pipette. Hold the urine pipette upright about 1/2 inch above the sample well of the device. Add 5 drops of urine to this well.
3. Point the absorbent tip downward, place the absorbent tip in the urine stream for at least 5 seconds to be thoroughly wet. Alternately, you can collect your urine into a clean cup and dip half of the absorbent pad into the urine for at least 5 seconds.
INTERPRETATION OF RESULTS
No LH Surge: Only one color band appears on the control (C) region or the test (T) band appears but is lighter than the control band. There is no LH surge detected.
LH Surge: If two color bands are visible and the test (T) band is equal to or darker than the control (C) band, one will probably ovulate in the next 24-48 hours. If one wants to be pregnant, the best time to have intercourse is after 24 but before 48 hours.
Invalid: No visible band at all. Repeat with a new test kit.
